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Bulletin of Osaka Prefecture University. Ser. B, Agriculture and life sciences >
Vol.50 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10466/3075

Title: A Two-Dimensional Electrophoretic Analysis of Serum Proteins of Rainbow Trout (Oncorhynchus mykiss) Treated with Inflammation Inducers
Authors: MURATA, Mie
SUGIMOTO, Chihiro
KODAMA, Hiroshi
ONUMA, Misao
Keywords: Acute phase protein
Rainbow trout
Two-dimensional electrophoresis
Issue Date: 31-Mar-1998
Publisher: Osaka Prefecture University
Citation: Bulletin of Osaka Prefecture University. Ser. B, Agriculture and life sciences. 1998, 50, p.59-65
Abstract: Changes in serum proteins of rainbow trout (Oncorhynchus mykiss) treated with inflammation inducers or exposed to high temperature were analysed by using two-dimensional gel electrophoresis employing isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis. Fishes were inoculated with lipopolysaccharide (LPS) or Freund's complete adjuvant (FCA) as inflammation inducer, or exposed to water temperature shift from 12℃ to 25℃. Increase of a protein of which apparent molecular weight of 43,000 and isoelectric point of 6.8 to 7.0 (43 kDa protein) was found in serum of fish inoculated with LPS or FCA. Contrary to this, few changes in serum protein compositions were observed in fish exposed to high temperature. The results suggest that the 43 kDa protein induced by LPS or FCA is a possible acute phase protein of rainbow trout.
URI: http://hdl.handle.net/10466/3075
Appears in Collections:Vol.50

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