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Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and biology >
Vol.23 >

このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/10466/3006

タイトル: Biological and Physical Properties of Tobacco Mosaic Virus Mutant Induced by Nitrous Acid
著者: TAKAHASHI, Minoru
OSAKI, Takeshi
発行日: 1971年3月31日
出版者: University of Osaka Prefecture
引用: Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and biology. 1971, 23, p.34-42
抄録: A mutant of tobacco mosaic virus, TMV-mutant 2-8, was isolated by selecting serially small local lesions on Nicotiana glutinosa leaves inoculated with the ordinary strain of tobacco mosaic virus (TMV-OM) that had been treated with nitrous acid. In tobacco (N. tabacum cv. Xanthi), the mutant 2-8 induced a very mild green mottle symptom. In tomato (Lycopersicon esculentum cv. Oogatahukuzyu), the virus apparently induced no symptoms, although the virus recovered in the infectivity assay on N. glutinosa leaves. Petunia (Petunia hybrida) was infected systemically showing a mild green mottle with a faint ruffle symptom or a mild green mottle with a slight leaf roll symptom. .The virus lost its infectivity at dilution about 5×10^5-10^6, and was inactivated by the following treatments: 5 minutes treatment at 90℃, 10 minutes at the range of 85-90℃, and 20 minutes at 85℃. The infectivity of the virus was lowered up to 14 per cent of TMV-OM after 32 days, when each virus was kept in the laboratory at room temperature. The virus was more resistant to UV irradiation than TMV-OM. The concentration of the virus in N. tabacum cv. Xanthi leaves was always lower than that of TMV-OM 24-72 hrs after inoculation. Tomato inoculated with the mutant 2-8 was completely protected against reinoculation with TMV-OM.
URI: http://hdl.handle.net/10466/3006


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