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Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and biology >
Vol.17 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10466/2946

Title: Ecologic and Taxonomic Studies on Pythium as Pathogenic Soil Fungi : VI The Isolation Methods of Pythium
Authors: TAKAHASHI, Minoru
OZAKI, Takeshi
Issue Date: 31-Mar-1965
Publisher: University of Osaka Prefecture
Citation: Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and biology. 1965, 17, p.1-10
Abstract: Selective media for the isolation of Pythium such as P. ultimum, P. aphanidermatum, P. spinosum, P. vexans, and P. zingiberum, and other soil-borne pathogens like Fusarium oxysporum f. lycopersici, Rhizoctonia solani and Sclerotium rolfsii were found. The media found ideal for the isolation of the specific organisms free from bacterial contamination as well as other fungi are the CMA-S50-A50, CMA-S50-P1000, PDA-S50-RB30-P300 and CMA-S50-P500. Streptomycin and penicillin at 50 ppm have been found to reduce bacterial contamination without inhibiting growth of Pythium. Moreover, PCNB at a concentration of 1000ppm in corn-meal agar eliminates other fungi without adversely affecting the development of Pythium. MARTIN's peptone-dextrose-rose bengal agar with 500 ppm PCNB has been found also to reduce greatly the number of contaminating fungi and actinomycetes. Pythium aphanidermatum, P. spinosum, P. ultimum, P. vexans, and P. zingiberum were isolated from bacteria, actinomycetes and other soil-borne fungi by using corn-meal agar containing 50 ppm streptomycin and 1000 ppm PCNB. Peptone-dextrose agar containing 30 ppm rose bengal, 50 ppm streptomycin and 500 ppm PCNB are also effective media for the isolation of Pythium from soil-borne microorganisms.
URI: http://hdl.handle.net/10466/2946
Appears in Collections:Vol.17

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