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Please use this identifier to cite or link to this item: http://hdl.handle.net/10466/2464

Title: 胃癌由来培養細胞による補体D因子産生(自然科学系)
Other Titles: Synthesis of Complement Factor D by Gastric Cancer-Derived Cell Lines
Authors: 北野, 悦子
北村, 肇
Author's alias: Kitano, Etsuko
Kitamura, Hajime
Keywords: factor D
complement
complement synthesis
gastric cancer-derived cell line
Issue Date: 31-Dec-1997
Publisher: 大阪府立看護大学医療技術短期大学部
Citation: 大阪府立看護大学医療技術短期大学部紀要. 1997, 3, p.23-29
Abstract: Factor D, a serum protein of the alternative pathway of the complement system, was assayed by sandwich ELISA in culture supernatants of 4 kinds of gastric cancer-derived cell lines, MKN28, MKN74, MKN45 and KATO-III. When these cells were cultured in protein-free hybridoma medium for 3 days without addition of any stimulator, 0.918±0.987, 1.953±0.489, 7.096±4.273 and 2.041±0.786 ng factor D/l0^6 cells were detected in the culture supernatants of MKN28, MKN74, MKN45 and KATO-III, respectively. Addition of TNF to the culture medium did not affect the factor D level significantly, while the C3 level increased in a dose-dependent manner. Factor D production by these cells was inhibited by the presence of 1.0 μg/mL of cycloheximide reversibly, indicating de novo synthesis of factor D by these cells. Since all of the cell lines tested in this study synthesize factor D, it is possible that factor D is synthesized by gastric epithelial cells physiologically. This is the first report on factor D synthesis by gastric epithelial cells.
URI: http://hdl.handle.net/10466/2464
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